G898
miRNA All-In-One cDNA Synthesis Kit
20 rxn
Reverse Transcriptase & RT-PCR

Description:
The miRNA All-In-One cDNA Synthesis Kit provides a comprehensive solution for efficient synthesis of first-strand miRNA cDNA from RNA samples. The kit includes all necessary components for a hassle-free setup, enabling RNA molecules to undergo polyadenylation and reverse transcription in a simple, single-tube reaction. This process uses Poly(A) Polymerase, OneScript® Plus Reverse Transcriptase, and a modified Oligo d(T) with Adapter that anneals to poly(A)-tailed miRNA. The included SuperMix contains RNaseOFF Ribonuclease Inhibitor to protect RNA from degradation.
 
The synthesized first-strand miRNA cDNA can be directly used as a template for qPCR-based miRNA expression analysis with BlasTaq™ qPCR MasterMix (sold separately, Cat. No. G891). Users can choose from abm’s extensive collection of miRNA qPCR primers, covering all known miRNA sequences. Each primer set includes a unique miRNA Forward Primer and a Universal 3' miRNA Reverse Primer that binds to the complementary Adapter sequence on the modified Oligo d(T).